9E9F
Pseudomonas aeruginosa MexY
Summary for 9E9F
| Entry DOI | 10.2210/pdb9e9f/pdb |
| EMDB information | 47796 |
| Descriptor | Efflux pump membrane transporter (1 entity in total) |
| Functional Keywords | pseudomonsa aeruginosa, mexy, rnd, efflux, membrane protein |
| Biological source | Pseudomonas aeruginosa |
| Total number of polymer chains | 3 |
| Total formula weight | 333863.74 |
| Authors | Gregor, W.D.,Maharjan, R.,Zhang, Z.,Yu, E.W. (deposition date: 2024-11-08, release date: 2024-12-18, Last modification date: 2026-01-21) |
| Primary citation | Gregor, W.D.,Maharjan, R.,Zhang, Z.,Chiaraviglio, L.,Sastry, N.,Cui, M.,Kirby, J.E.,Yu, E.W. Cryo-EM structure of the Pseudomonas aeruginosa MexY multidrug efflux pump. Mbio, 16:e0382624-e0382624, 2025 Cited by PubMed Abstract: , a Gram-negative pathogen, has emerged as one of the most highly antibiotic-resistant bacteria worldwide and subsequently has become a leading cause of healthcare-associated, life-threatening infections. multidrug efflux Y (MexY) is an efflux pump that belongs to the resistance-nodulation-cell division (RND) superfamily. It is a major determinant for resistance to aminoglycosides in this opportunistic pathogen. However, the detailed molecular mechanisms involved in aminoglycoside recognition and extrusion by MexY have not been elucidated. Here, we report the cryo-electron microscopy structure of MexY to a resolution of 3.63 Å. The structure directly indicates two plausible pathways for drug export. It also suggests that MexY is capable of picking up antibiotics via the ceiling of the central cavity formed by the MexY trimer. Molecular dynamics simulations depict that MexY is able to use a tunnel connecting the central cavity to the funnel of the trimer to export its substrates. PubMed: 40042268DOI: 10.1128/mbio.03826-24 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.63 Å) |
Structure validation
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