9CP0

Crystal structure of the porcine astrovirus 4 capsid spike domain

Summary for 9CP0

• Entry DOI: 10.2210/pdb9cp0/pdb
• Descriptor: Spike protein, CALCIUM ION (3 entities in total)
• Functional Keywords: porcine astrovirus 4, capsid, spike, viral protein
• Biological source: Porcine astrovirus 4
• Total number of polymer chains: 4
• Total formula weight: 108610.12

Authors

Haley, D.J.,DuBois, R.M. (deposition date: 2024-07-17, release date: 2024-10-23, Last modification date: 2024-11-13)

Primary citation

Haley, D.J.,Lanning, S.,Henricson, K.E.,Mardirossian, A.A.,Cirillo, I.,Rahe, M.C.,DuBois, R.M.
Structure and Antigenicity of the Porcine Astrovirus 4 Capsid Spike.
Viruses, 16:-, 2024

PubMed Abstract: Porcine astrovirus 4 (PoAstV4) has been recently associated with respiratory disease in pigs. In order to understand the scope of PoAstV4 infections and to support the development of a vaccine to combat PoAstV4 disease in pigs, we designed and produced a recombinant PoAstV4 capsid spike protein for use as an antigen in serological assays and for potential future use as a vaccine antigen. Structural prediction of the full-length PoAstV4 capsid protein guided the design of the recombinant PoAstV4 capsid spike domain expression plasmid. The recombinant PoAstV4 capsid spike was expressed in , purified by affinity and size-exclusion chromatography, and its crystal structure was determined at 1.85 Å resolution, enabling structural comparisons to other animal and human astrovirus capsid spike structures. The recombinant PoAstV4 capsid spike protein was also used as an antigen for the successful development of a serological assay to detect PoAstV4 antibodies, demonstrating that the recombinant PoAstV4 capsid spike retains antigenic epitopes found on the native PoAstV4 capsid. These studies lay a foundation for seroprevalence studies and the development of a PoAstV4 vaccine for swine.

PubMed: 39459929
DOI: 10.3390/v16101596

Experimental method

X-RAY DIFFRACTION (1.85 Å)