9CP0
Crystal structure of the porcine astrovirus 4 capsid spike domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-11-11 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 |
Unit cell lengths | 46.386, 66.211, 87.752 |
Unit cell angles | 74.54, 81.67, 78.61 |
Refinement procedure
Resolution | 39.780 - 1.850 |
R-factor | 0.3175 |
Rwork | 0.316 |
R-free | 0.36170 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.743 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.780 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 80526 | 3920 |
<I/σ(I)> | 8.1 | 2.1 |
Completeness [%] | 96.1 | |
Redundancy | 3.2 | |
CC(1/2) | 0.984 | 0.814 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 0.2 M calcium acetate hydrate, 0.1 M Tris-Cl pH 7, and 12% PEG 3000 |