9CE3

Structure of the TSC:WIPI3 lysosomal recruitment complex

Summary for 9CE3

• Entry DOI: 10.2210/pdb9ce3/pdb
• Related: 9CE3
• EMDB information: 45492
• Descriptor: Isoform 4 of Tuberin, Hamartin, TBC1 domain family member 7, ... (5 entities in total)
• Functional Keywords: tuberous sclerosis complex, tumour suppressor, gtpase-activating proteins (gap), tsc-mtorc pathway, oncoprotein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 8
• Total formula weight: 736998.63

Authors

Bayly-Jones, C.,Lupton, C.J.,D'Andrea, L.,Ellisdon, A.M. (deposition date: 2024-06-26, release date: 2024-12-04)

Primary citation

Bayly-Jones, C.,Lupton, C.J.,D'Andrea, L.,Chang, Y.G.,Jones, G.D.,Steele, J.R.,Venugopal, H.,Schittenhelm, R.B.,Halls, M.L.,Ellisdon, A.M.
Structure of the human TSC:WIPI3 lysosomal recruitment complex.
Sci Adv, 10:eadr5807-eadr5807, 2024

PubMed Abstract: Tuberous sclerosis complex (TSC) is targeted to the lysosomal membrane, where it hydrolyzes RAS homolog-mTORC1 binding (RHEB) from its GTP-bound to GDP-bound state, inhibiting mechanistic target of rapamycin complex 1 (mTORC1). Loss-of-function mutations in TSC cause TSC disease, marked by excessive tumor growth. Here, we overcome a high degree of continuous conformational heterogeneity to determine the 2.8-Å cryo-electron microscopy (cryo-EM) structure of the complete human TSC in complex with the lysosomal recruitment factor WD repeat domain phosphoinositide-interacting protein 3 (WIPI3). We discover a previously undetected amino-terminal TSC1 HEAT repeat dimer that clamps onto a single TSC wing and forms a phosphatidylinositol phosphate (PIP)-binding pocket, which specifically binds monophosphorylated PIPs. These structural advances provide a model by which WIPI3 and PIP-signaling networks coordinate to recruit TSC to the lysosomal membrane to inhibit mTORC1. The high-resolution TSC structure reveals previously unrecognized mutational hotspots and uncovers crucial insights into the mechanisms of TSC dysregulation in disease.

PubMed: 39565846
DOI: 10.1126/sciadv.adr5807

Experimental method

ELECTRON MICROSCOPY (2.9 Å)