9CAI

High-resolution C. elegans 80S ribosome structure - class 1

これはPDB形式変換不可エントリーです。

9CAI の概要

• エントリーDOI: 10.2210/pdb9cai/pdb
• 関連するPDBエントリー: 9BH5
• EMDBエントリー: 45392
• 分子名称: Small ribosomal subunit protein uS2, 5.8S rRNA, 18S rRNA, ... (81 entities in total)
• 機能のキーワード: ribosome, endogenous purified 80s ribosome
• 由来する生物種: Caenorhabditis elegans; 詳細
• タンパク質・核酸の鎖数: 79
• 化学式量合計: 3163653.10

構造登録者

Sehgal, E.,Serrao, V.H.B.,Arribere, J. (登録日: 2024-06-17, 公開日: 2024-09-11, 最終更新日: 2024-10-30)

主引用文献

Sehgal, E.,Wohlenberg, C.,Soukup, E.M.,Viscardi, M.J.,Serrao, V.H.B.,Arribere, J.A.
High-resolution reconstruction of a C. elegans ribosome sheds light on evolutionary dynamics and tissue specificity.
Rna, 30:1513-1528, 2024

PubMed Abstract: is an important model organism for human health and disease, with foundational contributions to the understanding of gene expression and tissue patterning in animals. An invaluable tool in modern gene expression research is the presence of a high-resolution ribosome structure, though no such structure exists for Here, we present a high-resolution single-particle cryogenic electron microscopy (cryo-EM) reconstruction and molecular model of a ribosome, revealing a significantly streamlined animal ribosome. Many facets of ribosome structure are conserved in , including overall ribosomal architecture and the mechanism of cycloheximide, whereas other facets, such as expansion segments and eL28, are rapidly evolving. We identify uL5 and uL23 as two instances of tissue-specific ribosomal protein paralog expression conserved in , suggesting that ribosomes vary across tissues. The ribosome structure will provide a basis for future structural, biochemical, and genetic studies of translation in this important animal system.

PubMed: 39209556
DOI: 10.1261/rna.080103.124

実験手法

ELECTRON MICROSCOPY (2.59 Å)