9C1B

Crystal structure of GDP-bound human M-RAS protein in crystal form II

9C1B の概要

• エントリーDOI: 10.2210/pdb9c1b/pdb
• 関連するPDBエントリー: 9C1A
• 分子名称: Ras-related protein M-Ras, MAGNESIUM ION, GUANOSINE-5'-DIPHOSPHATE, ... (7 entities in total)
• 機能のキーワード: m-ras, gdp, gtpase structure, ras, crystal packing, hydrolase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 103964.68

構造登録者

Bester, S.M.,Abrahamsen, R.,Samora, L.R.,Wu, W.-I.,Mou, T.-C. (登録日: 2024-05-28, 公開日: 2024-09-11, 最終更新日: 2024-09-18)

主引用文献

Bester, S.M.,Abrahamsen, R.,Rodrigues Samora, L.,Wu, W.I.,Mou, T.C.
Crystal structure of the GDP-bound human M-RAS protein in two crystal forms.
Acta Crystallogr.,Sect.F, 80:220-227, 2024

PubMed Abstract: M-RAS plays a crucial role in the RAF-MEK signaling pathway. When activated by GTP, M-RAS forms a complex with SHOC2 and PP1C, initiating downstream RAF-MEK signal transduction. In this study, the crystal structure of the GDP-bound human M-RAS protein is presented with two forms of crystal packing. Both the full-length and truncated human M-RAS structures aligned well with the high-confidence section of the AlphaFold2-predicted structure with low r.m.s.d., except for the Switch regions. Despite high sequence similarity to the available mouse M-RAS structure, the full-length human M-RAS structure exhibits unique crystal packing. This inactive human M-RAS structure could offer novel insights for the design of selective compounds targeting M-RAS.

PubMed: 39196705
DOI: 10.1107/S2053230X24007969

実験手法

X-RAY DIFFRACTION (2.27 Å)