9BRZ

V0-only V-ATPase and synaptophysin complex in mouse brain isolated synaptic vesicles

Summary for 9BRZ

• Entry DOI: 10.2210/pdb9brz/pdb
• EMDB information: 44846
• Descriptor: V-type proton ATPase subunit S1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (12 entities in total)
• Functional Keywords: v0only v-atpase, synaptic vesicle, membrane protein
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 17
• Total formula weight: 447733.34

Authors

Wang, C.,Jiang, W.,Yang, K.,Wang, X.,Guo, Q.,Brunger, A.T. (deposition date: 2024-05-12, release date: 2024-06-19, Last modification date: 2024-11-13)

Primary citation

Wang, C.,Jiang, W.,Leitz, J.,Yang, K.,Esquivies, L.,Wang, X.,Shen, X.,Held, R.G.,Adams, D.J.,Basta, T.,Hampton, L.,Jian, R.,Jiang, L.,Stowell, M.H.B.,Baumeister, W.,Guo, Q.,Brunger, A.T.
Structure and topography of the synaptic V-ATPase-synaptophysin complex.
Nature, 631:899-904, 2024

PubMed Abstract: Synaptic vesicles are organelles with a precisely defined protein and lipid composition, yet the molecular mechanisms for the biogenesis of synaptic vesicles are mainly unknown. Here, we discovered a well-defined interface between the synaptic vesicle V-ATPase and synaptophysin by in situ cryo-electron tomography and single particle cryo-electron microscopy of functional synaptic vesicles isolated from mouse brains. The synaptic vesicle V-ATPase is an ATP-dependent proton pump that establishes the protein gradient across the synaptic vesicle, which in turn drives the uptake of neurotransmitters. Synaptophysin and its paralogs synaptoporin and synaptogyrin belong to a family of abundant synaptic vesicle proteins whose function is still unclear. We performed structural and functional studies of synaptophysin knockout mice, confirming the identity of synaptophysin as an interaction partner with the V-ATPase. Although there is little change in the conformation of the V-ATPase upon interaction with synaptophysin, the presence of synaptophysin in synaptic vesicles profoundly affects the copy number of V-ATPases. This effect on the topography of synaptic vesicles suggests that synaptophysin assists in their biogenesis. In support of this model, we observed that synaptophysin knockout mice exhibit severe seizure susceptibility, suggesting an imbalance of neurotransmitter release as a physiological consequence of the absence of synaptophysin.

PubMed: 38838737
DOI: 10.1038/s41586-024-07610-x

Experimental method

ELECTRON MICROSCOPY (3.8 Å)