9BGL

Complex of Zinc Finger Antiviral Protein RBD and KHNYN CTD

Summary for 9BGL

• Entry DOI: 10.2210/pdb9bgl/pdb
• Descriptor: Zinc finger CCCH-type antiviral protein 1, Protein KHNYN, ZINC ION, ... (5 entities in total)
• Functional Keywords: zinc finger antiviral protein, zap, rna binding domain, antiviral protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 33075.77

Authors

Meagher, J.L.,Smith, J.L. (deposition date: 2024-04-19, release date: 2025-01-22)

Primary citation

Bohn, J.A.,Meagher, J.L.,Takata, M.A.,Goncalves-Carneiro, D.,Yeoh, Z.C.,Ohi, M.D.,Smith, J.L.,Bieniasz, P.D.
Functional anatomy of zinc finger antiviral protein complexes.
Nat Commun, 15:10834-10834, 2024

PubMed Abstract: ZAP is an antiviral protein that binds to and depletes viral RNA, which is often distinguished from vertebrate host RNA by its elevated CpG content. Two ZAP cofactors, TRIM25 and KHNYN, have activities that are poorly understood. Here, we show that functional interactions between ZAP, TRIM25 and KHNYN involve multiple domains of each protein, and that the ability of TRIM25 to multimerize via its RING domain augments ZAP activity and specificity. We show that KHNYN is an active nuclease that acts in a partly redundant manner with its homolog N4BP1. The ZAP N-terminal RNA binding domain constitutes a minimal core that is essential for antiviral complex activity, and we present a crystal structure of this domain that reveals contacts with the functionally required KHNYN C-terminal domain. These contacts are remote from the ZAP CpG binding site and would not interfere with RNA binding. Based on our dissection of ZAP, TRIM25 and KHNYN functional anatomy, we could design artificial chimeric antiviral proteins that reconstitute the antiviral function of the intact authentic proteins, but in the absence of protein domains that are otherwise required for activity. Together, these results suggest a model for the RNA recognition and action of ZAP-containing antiviral protein complexes.

PubMed: 39738020
DOI: 10.1038/s41467-024-55192-z

Experimental method

X-RAY DIFFRACTION (2.294 Å)