9BGL

Complex of Zinc Finger Antiviral Protein RBD and KHNYN CTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2020-08-11
Detector	MAR CCD 300 mm
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	36.213, 88.001, 94.546
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.000 - 2.294
R-factor	0.2082
Rwork	0.206
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.850
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (23-JAN-2024))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	44.000	2.340
High resolution limit [Å]	2.294	6.240	2.300
Rmerge	0.065	0.041	0.548
Rmeas	0.070	0.045	0.591
Rpim	0.026	0.018	0.219
Number of reflections	14154	801	685
<I/σ(I)>	8.5
Completeness [%]	100.0	99.4	100
Redundancy	7.1	6.1	7.2
CC(1/2)	1.000	0.997	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350, 0.2M NaCl