9B8R
Cryo-EM structure of S. cerevisiae PolE-core-DNA
Summary for 9B8R
| Entry DOI | 10.2210/pdb9b8r/pdb |
| EMDB information | 44356 |
| Descriptor | Primer DNA, Template DNA, DNA polymerase epsilon catalytic subunit, ... (4 entities in total) |
| Functional Keywords | pol2, dna, replication, dna binding protein-dna complex, dna binding protein/dna |
| Biological source | Saccharomyces cerevisiae (brewer's yeast) More |
| Total number of polymer chains | 3 |
| Total formula weight | 140957.08 |
| Authors | Yuan, Z.,Georgescu, R.,O'Donnell, M.,Li, H. (deposition date: 2024-03-31, release date: 2024-10-09, Last modification date: 2024-10-23) |
| Primary citation | Yuan, Z.,Georgescu, R.,Yao, N.Y.,Yurieva, O.,O'Donnell, M.E.,Li, H. Mechanism of PCNA loading by Ctf18-RFC for leading-strand DNA synthesis. Science, 385:eadk5901-eadk5901, 2024 Cited by PubMed Abstract: The proliferating cell nuclear antigen (PCNA) clamp encircles DNA to hold DNA polymerases (Pols) to DNA for processivity. The Ctf18-RFC PCNA loader, a replication factor C (RFC) variant, is specific to the leading-strand Pol (Polε). We reveal here the underlying mechanism of Ctf18-RFC specificity to Polε using cryo-electron microscopy and biochemical studies. We found that both Ctf18-RFC and Polε contain specific structural features that direct PCNA loading onto DNA. Unlike other clamp loaders, Ctf18-RFC has a disordered ATPase associated with a diverse cellular activities (AAA+) motor that requires Polε to bind and stabilize it for efficient PCNA loading. In addition, Ctf18-RFC can pry prebound Polε off of DNA, then load PCNA onto DNA and transfer the PCNA-DNA back to Polε. These elements in both Ctf18-RFC and Polε provide specificity in loading PCNA onto DNA for Polε. PubMed: 39088616DOI: 10.1126/science.adk5901 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.5 Å) |
Structure validation
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