9ATZ
HIV 16055.v8.3 SOSIP Env in Complex with V2 Epitope and Anti-Immune Complex pAbs from Rabbit 2464
This is a non-PDB format compatible entry.
Summary for 9ATZ
| Entry DOI | 10.2210/pdb9atz/pdb |
| EMDB information | 43838 |
| Descriptor | Rabbit V2 Polyclonal Antibody - Predicted Light Chain, Rabbit Anti-Immune Complex Antibody - Predicted Light Chain, Rabbit V2 Polyclonal Antibody - Predicted Heavy Chain, ... (9 entities in total) |
| Functional Keywords | hiv, polyclonal, antibodies, cryoempem, rabbit, anti-immune complex, v1/v3, viral protein |
| Biological source | Human immunodeficiency virus 1 More |
| Total number of polymer chains | 18 |
| Total formula weight | 358489.68 |
| Authors | |
| Primary citation | Brown, S.,Antanasijevic, A.,Sewall, L.M.,Garcia, D.M.,Brouwer, P.J.M.,Sanders, R.W.,Ward, A.B. Anti-immune complex antibodies are elicited during repeated immunization with HIV Env immunogens. Sci Immunol, 10:eadp5218-eadp5218, 2025 Cited by PubMed Abstract: Vaccination strategies against HIV-1 aim to elicit broadly neutralizing antibodies (bnAbs) using prime-boost regimens with HIV envelope (Env) immunogens. Epitope mapping has shown that early antibody responses are directed to easily accessible nonneutralizing epitopes on Env instead of bnAb epitopes. Autologously neutralizing antibody responses appear upon boosting, once immunodominant epitopes are saturated. Here, we use electron microscopy-based polyclonal epitope mapping (EMPEM) to elucidate how repeated immunization with HIV Env SOSIP immunogens results in the generation of Ab2α anti-idiotypic antibodies in rabbits and rhesus macaques. We present the structures of six anti-immune complex antibodies and find that they target idiotopes composed of framework regions of antibodies bound to Env. Examination of cryo-electron microscopy density enabled prediction of sequences for an anti-immune complex antibody, the paratope of which is enriched with aromatic amino acids. This work sheds light on current vaccine development efforts for HIV, as well as for other pathogens in which repeated exposure to antigen is required. PubMed: 39823319DOI: 10.1126/sciimmunol.adp5218 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.4 Å) |
Structure validation
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