9AR6

CryoEM structure of ThermoCas9 in pre-cleavage state bound with the DNA containing NNNNCCA PAM

Summary for 9AR6

• Entry DOI: 10.2210/pdb9ar6/pdb
• EMDB information: 43771
• Descriptor: CRISPR-associated endonuclease Cas9, non-target DNA, target-strand DNA, ... (6 entities in total)
• Functional Keywords: crispr-cas9; cas9; epigenetics; endonuclease; hnh; ruvc; metal binding; rna, dna binding protein-dna-rna complex, dna binding protein/dna/rna
• Biological source: Geobacillus thermodenitrificans; More
• Total number of polymer chains: 4
• Total formula weight: 189626.42

Authors

Zhao, Y.,Shu, Y.,Li, H. (deposition date: 2024-02-22, release date: 2025-02-26)

• Primary citation: Roth, M.,Shu, Y.,Trasanidou, D.,Zhao, Y.,van der Oost, J.,Li, H.; Molecular Basis for Epigenetic-sensitive Editing by Cas9; To be Published,

Experimental method

ELECTRON MICROSCOPY (2.77 Å)