9AR6
CryoEM structure of ThermoCas9 in pre-cleavage state bound with the DNA containing NNNNCCA PAM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9 | polymer | 1082 | 126563.5 | 1 | UniProt (A0A1W6VMQ3) | Geobacillus thermodenitrificans | |
| 2 | B (D) | non-target DNA | polymer | 12 | 3615.4 | 1 | Geobacillus thermodenitrificans | ||
| 3 | C (P) | target-strand DNA | polymer | 40 | 12238.9 | 1 | Geobacillus thermodenitrificans | ||
| 4 | D (B) | single guide RNA | polymer | 147 | 47162.7 | 1 | Geobacillus thermodenitrificans | ||
| 5 | E, F (B) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 6 | G, H (P, B) | water | water | 18.0 | 2 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 189580.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 46.0 | |
| All* | Total formula weight | 189626.4 |
