9AR4

CryoEM structure of ThermoCas9 in post-cleavage state bound with the DNA containing NNNNCCA PAM

Summary for 9AR4

• Entry DOI: 10.2210/pdb9ar4/pdb
• EMDB information: 43769
• Descriptor: Single guide RNA, Cleaved 3' target DNA strand, Cleaved 3' non-target DNA strand, ... (9 entities in total)
• Functional Keywords: crispr-cas9; cas9; epigenetics; endonuclease; hnh; ruvc; metal binding; rna, dna binding protein-dna-rna complex, dna binding protein/dna/rna
• Biological source: Geobacillus thermodenitrificans; More
• Total number of polymer chains: 6
• Total formula weight: 192977.30

Authors

Shu, Y.,Zhao, Y.,Li, H. (deposition date: 2024-02-22, release date: 2025-02-26)

• Primary citation: Roth, M.,Shu, Y.,Trasanidis, D.,Zhao, Y.,Hoffman, R.,van der Oost, J.,Li, H.; Molecular Basis for Epigenetic-sensitive Editing by Cas9; To Be Published,

Experimental method

ELECTRON MICROSCOPY (2.2 Å)