9AR4

CryoEM structure of ThermoCas9 in post-cleavage state bound with the DNA containing NNNNCCA PAM

Summary for 9AR4

Entry DOI	10.2210/pdb9ar4/pdb
EMDB information	43769
Descriptor	Single guide RNA, Cleaved 3' target DNA strand, Cleaved 3' non-target DNA strand, ... (9 entities in total)
Functional Keywords	crispr-cas9; cas9; epigenetics; endonuclease; hnh; ruvc; metal binding; rna, dna binding protein-dna-rna complex, dna binding protein/dna/rna
Biological source	Geobacillus thermodenitrificans More
Total number of polymer chains	6
Total formula weight	192977.30
Authors	Shu, Y.,Zhao, Y.,Li, H. (deposition date: 2024-02-22, release date: 2025-02-26, Last modification date: 2026-04-15)
Primary citation	Roth, M.O.,Shu, Y.,Zhao, Y.,Trasanidou, D.,Hoffman, R.D.,Trasanidis, N.,Gelasco, M.K.,Medina, M.L.,Das, D.,Rai, J.,Wang, B.,Li, H. Molecular basis for methylation-sensitive editing by Cas9 Nature, 2026 Cited by DOI: 10.1038/s41586-026-10384-z PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (2.2 Å)

Structure validation