9AR4
CryoEM structure of ThermoCas9 in post-cleavage state bound with the DNA containing NNNNCCA PAM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Single guide RNA | polymer | 149 | 47798.1 | 1 | Geobacillus thermodenitrificans | ||
| 2 | B (C) | Cleaved 3' target DNA strand | polymer | 26 | 7883.1 | 1 | synthetic construct | ||
| 3 | C (D) | Cleaved 3' non-target DNA strand | polymer | 14 | 4248.8 | 1 | synthetic construct | ||
| 4 | D (P) | Cleaved 5' target DNA strand | polymer | 14 | 4310.8 | 1 | synthetic construct | ||
| 5 | E (X) | Cleaved 5' non-target DNA strand | polymer | 5 | 1504.0 | 1 | synthetic construct | ||
| 6 | F (A) | CRISPR-associated endonuclease Cas9 | polymer | 1082 | 126563.5 | 1 | UniProt (A0A1W6VMQ3) | Geobacillus thermodenitrificans | |
| 7 | G, H, J, K, L... (B, A) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
| 8 | I (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 9 | N, O, P (B, C, A) | water | water | 18.0 | 21 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 192308.3 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 669.0 | |
| All* | Total formula weight | 192977.3 |
