9AR4
CryoEM structure of ThermoCas9 in post-cleavage state bound with the DNA containing NNNNCCA PAM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Single guide RNA | polymer | 149 | 47798.1 | 1 | Geobacillus thermodenitrificans | ||
| 2 | B (C) | Cleaved 3' target DNA strand | polymer | 26 | 7883.1 | 1 | synthetic construct | ||
| 3 | C (D) | Cleaved 3' non-target DNA strand | polymer | 14 | 4248.8 | 1 | synthetic construct | ||
| 4 | D (P) | Cleaved 5' target DNA strand | polymer | 14 | 4310.8 | 1 | synthetic construct | ||
| 5 | E (X) | Cleaved 5' non-target DNA strand | polymer | 5 | 1504.0 | 1 | synthetic construct | ||
| 6 | F (A) | CRISPR-associated endonuclease Cas9 | polymer | 1082 | 126563.5 | 1 | UniProt (A0A1W6VMQ3) | Geobacillus thermodenitrificans | |
| 7 | G, H, J, K, L... (B, A) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) PubChem (888) | |||
| 8 | I (C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 9 | N, O, P (B, C, A) | water | water | 18.0 | 21 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 192308.3 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 669.0 | |
| All* | Total formula weight | 192977.3 |
