8ZU3

Human PIEZO1-MDFIC

Summary for 8ZU3

• Entry DOI: 10.2210/pdb8zu3/pdb
• EMDB information: 60479
• Descriptor: Piezo-type mechanosensitive ion channel component 1, MyoD family inhibitor domain-containing protein, DODECANE, ... (4 entities in total)
• Functional Keywords: human piezo1, membrane protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 6
• Total formula weight: 943517.70

Authors

Zhang, M.F. (deposition date: 2024-06-07, release date: 2025-01-15, Last modification date: 2025-08-27)

Primary citation

Shan, Y.,Guo, X.,Zhang, M.,Chen, M.,Li, Y.,Zhang, M.,Pei, D.
Structure of human PIEZO1 and its slow-inactivating channelopathy mutants.
Elife, 13:-, 2025

PubMed Abstract: PIEZO channels transmit mechanical force signals to cells, allowing them to make critical decisions during development and in pathophysiological conditions. Their fast/slow inactivation modes have been implicated in mechanopathologies but remain poorly understood. Here, we report several near-atomic resolution cryo-EM structures of fast-inactivating wild-type human PIEZO1 (hPIEZO1) and its slow-inactivating channelopathy mutants with or without its auxiliary subunit MDFIC. Our results suggest that hPIEZO1 has a more flattened and extended architecture than curved mouse PIEZO1 (mPIEZO1). The multi-lipidated MDFIC subunits insert laterally into the hPIEZO1 pore module like mPIEZO1, resulting in a more curved and extended state. Interestingly, the high-resolution structures suggest that the pore lipids, which directly seal the central hydrophobic pore, may be involved in the rapid inactivation of hPIEZO1. While the severe hereditary erythrocytosis mutant R2456H significantly slows down the inactivation of hPIEZO1, the hPIEZO1-R2456H-MDFIC complex shows a more curved and contracted structure with an inner helix twist due to the broken link between the pore lipid and R2456H. These results suggest that the pore lipids may be involved in the mechanopathological rapid inactivation mechanism of PIEZO channels.

PubMed: 40668110
DOI: 10.7554/eLife.101923

Experimental method

ELECTRON MICROSCOPY (3.1 Å)