8ZNG

Cryo-EM structure of W89F mutated Glutamate dehydrogenase from Thermococcus profundus in complex with NADPH and AKG in the steady stage of reaction

Summary for 8ZNG

• Entry DOI: 10.2210/pdb8zng/pdb
• Related: 8ZMU
• EMDB information: 60270
• Descriptor: Glutamate dehydrogenase, NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, 2-OXOGLUTARIC ACID, ... (4 entities in total)
• Functional Keywords: oxidoreductase, complex, nadph, 2-oxoglutarate, mutant
• Biological source: Thermococcus profundus
• Total number of polymer chains: 1
• Total formula weight: 47251.52

Authors

Wakabayashi, T.,Nakasako, M. (deposition date: 2024-05-26, release date: 2024-06-12)

• Primary citation: Wakabayashi, T.,Oide, M.,Matsui, Y.,Nakasako, M.; Mechanism for drastic reduction in catalytic activity of Trp89Phe-mutated glutamate dehydrogenase revealed by crystal structure and cryoEM-sampling of metastable conformation in action; To Be Published,

Experimental method

ELECTRON MICROSCOPY (2.5 Å)