8ZNG
Cryo-EM structure of W89F mutated Glutamate dehydrogenase from Thermococcus profundus in complex with NADPH and AKG in the steady stage of reaction
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | JEOL CRYO ARM 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.5 |
Number of Particle | 3534400 |
Software | RELION,SerialEM,CTFFIND,PHENIX |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |