8Z70
State 1 (S1) of yeast 80S ribosome bound to 2 tRNAs during mRNA decoding
This is a non-PDB format compatible entry.
Summary for 8Z70
| Entry DOI | 10.2210/pdb8z70/pdb |
| EMDB information | 38656 |
| Descriptor | 18S rRNA, Small ribosomal subunit protein eS19A, Small ribosomal subunit protein uS10, ... (81 entities in total) |
| Functional Keywords | 80s, ribosome |
| Biological source | Saccharomyces cerevisiae S288C More |
| Total number of polymer chains | 81 |
| Total formula weight | 3126040.68 |
| Authors | Cheng, J.,Wu, C.L.,Li, J.X.,Zhang, X.Z. (deposition date: 2024-04-19, release date: 2024-12-11, Last modification date: 2025-07-02) |
| Primary citation | Cheng, J.,Wu, C.,Li, J.,Yang, Q.,Zhao, M.,Zhang, X. Capturing eukaryotic ribosome dynamics in situ at high resolution. Nat.Struct.Mol.Biol., 32:698-708, 2025 Cited by PubMed Abstract: Many protein complexes are highly dynamic in cells; thus, characterizing their conformational changes in cells is crucial for unraveling their functions. Here, using cryo-electron microscopy, 451,700 ribosome particles from Saccharomyces cerevisiae cell lamellae were obtained to solve the 60S region to 2.9-Å resolution by in situ single-particle analysis. Over 20 distinct conformations were identified by three-dimensional classification with resolutions typically higher than 4 Å. These conformations were used to reconstruct a complete elongation cycle of eukaryotic translation with elongation factors (eEFs). We found that compact eEF2 anchors to the partially rotated ribosome after subunit rolling and hypothesize that it stabilizes the local conformation for peptidyl transfer. Moreover, open-eEF3 binding to a fully rotated ribosome was observed, whose conformational change was coupled with head swiveling and body back-rotation of the 40S subunit. PubMed: 39789210DOI: 10.1038/s41594-024-01454-9 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.2 Å) |
Structure validation
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