8Z37
Crystal structure of human STING ligand binding domain.
Summary for 8Z37
| Entry DOI | 10.2210/pdb8z37/pdb |
| Descriptor | Stimulator of interferon genes protein, CHLORIDE ION, CALCIUM ION, ... (5 entities in total) |
| Functional Keywords | cgamp binding protein, immune system |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 1 |
| Total formula weight | 22354.56 |
| Authors | |
| Primary citation | Sun, P.,Wang, B.,Liu, C.,Wang, Z.,Liu, Y.,Qiao, Y.B.,Li, X. A fluorescent STING ligand sensor for high-throughput screening of compounds that can enhance tumor immunotherapy. Cell Rep Methods, 5:101106-101106, 2025 Cited by PubMed Abstract: The activation of the stimulator of interferon genes (STING) pathway triggers the release of type I interferons that can potentiate the host immune response against tumors. STING agonism is therefore a promising strategy for the development of cancer immunotherapy; however, sensitive tools and assays for the discovery of STING modulators are currently limited. Here, we develop and characterize a STING ligand sensor, FiSL, to detect STING ligands in vitro. Utilizing FiSL, we identify honokiol, a natural compound derived from Magnolia species, as an orally available STING agonist from a bioactive compound library. Functional studies reveal that honokiol exerts antitumor activity in a STING-dependent manner. Moreover, in STING-humanized mouse tumor models, honokiol enhances the efficacy of anti-PD-(L)1 immunotherapy. Collectively, we have developed FiSL as a tool for high-throughput screening of STING ligands and revealed honokiol as a STING agonist that can be harnessed to treat human cancer. PubMed: 40669456DOI: 10.1016/j.crmeth.2025.101106 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.4 Å) |
Structure validation
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