8YQH
Crystal structure of Cry3Aa-PEI at 3.1 A
Summary for 8YQH
| Entry DOI | 10.2210/pdb8yqh/pdb |
| Descriptor | Cry3Aa protein (2 entities in total) |
| Functional Keywords | bacillus thuringiensis, crystal protein, insecticidal protein, toxin |
| Biological source | Bacillus thuringiensis |
| Total number of polymer chains | 1 |
| Total formula weight | 73177.16 |
| Authors | |
| Primary citation | Yekta, R.,Xiong, X.,Li, J.,Heater, B.S.,Lee, M.M.,Chan, M.K. Mechanoresponsive Protein Crystals for NADH Recycling in Multicycle Enzyme Reactions. J.Am.Chem.Soc., 146:18817-18822, 2024 Cited by PubMed Abstract: NAD(H)-dependent enzymes play a crucial role in the biosynthesis of pharmaceuticals and fine chemicals, but the limited recyclability of the NAD(H) cofactor hinders its more general application. Here, we report the generation of mechano-responsive PEI-modified Cry3Aa protein crystals and their use for NADH recycling over multiple reaction cycles. For demonstration of its practical utility, a complementary Cry3Aa protein particle containing genetically encoded and co-immobilized formate dehydrogenase for NADH regeneration and leucine dehydrogenase for catalyzing the NADH-dependent l--leucine (l--Leu) biosynthesis has been produced. When combined with the PEI-modified Cry3Aa crystal, the resultant reaction system could be used for the efficient biosynthesis of l--Leu for up to 21 days with a 10.5-fold improvement in the NADH turnover number. PubMed: 38968608DOI: 10.1021/jacs.4c04725 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.11 Å) |
Structure validation
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