Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

8Y5G

Cryo-EM structure of E.coli spermidine transporter PotABC with spermidine

Summary for 8Y5G
Entry DOI10.2210/pdb8y5g/pdb
EMDB information38934
DescriptorSpermidine/putrescine import ATP-binding protein PotA, Spermidine/putrescine ABC transporter permease PotB, Spermidine/putrescine transport system permease protein PotC, ... (6 entities in total)
Functional Keywordsabc transporter, transport protein
Biological sourceEscherichia coli
More
Total number of polymer chains4
Total formula weight140426.30
Authors
Qiao, Z.,Gao, Y.G. (deposition date: 2024-01-31, release date: 2024-10-09)
Primary citationQiao, Z.,Do, P.H.,Yeo, J.Y.,Ero, R.,Li, Z.,Zhan, L.,Basak, S.,Gao, Y.G.
Structural insights into polyamine spermidine uptake by the ABC transporter PotD-PotABC.
Sci Adv, 10:eado8107-eado8107, 2024
Cited by
PubMed Abstract: Polyamines, characterized by their polycationic nature, are ubiquitously present in all organisms and play numerous cellular functions. Among polyamines, spermidine stands out as the predominant type in both prokaryotic and eukaryotic cells. The PotD-PotABC protein complex in , belonging to the adenosine triphosphate-binding cassette transporter family, is a spermidine-preferential uptake system. Here, we report structural details of the polyamine uptake system PotD-PotABC in various states. Our analyses reveal distinct "inward-facing" and "outward-facing" conformations of the PotD-PotABC transporter, as well as conformational changes in the "gating" residues (F222, Y223, D226, and K241 in PotB; Y219 and K223 in PotC) controlling spermidine uptake. Therefore, our structural analysis provides insights into how the PotD-PotABC importer recognizes the substrate-binding protein PotD and elucidates molecular insights into the spermidine uptake mechanism of bacteria.
PubMed: 39303029
DOI: 10.1126/sciadv.ado8107
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (3 Å)
Structure validation

227111

PDB entries from 2024-11-06

PDB statisticsPDBj update infoContact PDBjnumon