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8Y5G

Cryo-EM structure of E.coli spermidine transporter PotABC with spermidine

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)
Spermidine/putrescine import ATP-binding protein PotApolymer36041022.81UniProt (A0A2K3TLI6)
Pfam (PF00005)
Pfam (PF08402)
UniProt (by SIFTS) (P69874)
Escherichia coli
2B
(B)
Spermidine/putrescine ABC transporter permease PotBpolymer27330813.51UniProt (A0A3L9I213)
Pfam (PF00528)
UniProt (by SIFTS) (P0AFK4)
Escherichia coli
3C
(C)
Spermidine/putrescine transport system permease protein PotCpolymer24927527.01UniProt (C3TDI7)
Pfam (PF00528)
UniProt (by SIFTS) (P0AFK6)
Escherichia coli
4D
(D)
Spermidine/putrescine import ATP-binding protein PotApolymer35840820.61UniProt (A0A2K3TLI6)
Pfam (PF00005)
Pfam (PF08402)
UniProt (by SIFTS) (P69874)
Escherichia coli
5E, F, H, I
(A, D)
MAGNESIUM IONnon-polymer24.34Chemie (MG)
6G
(B)
SPERMIDINEnon-polymer145.21Chemie (SPD)
Sequence modifications
A: 15 - 374 (UniProt: A0A2K3TLI6)
PDBExternal DatabaseDetails
His 83Leu 83conflict
Gln 173Glu 173engineered mutation
Ala 283Gly 283conflict
D: 16 - 373 (UniProt: A0A2K3TLI6)
PDBExternal DatabaseDetails
His 83Leu 83conflict
Gln 173Glu 173engineered mutation
Ala 283Gly 283conflict
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains4
Total formula weight140183.8
Non-Polymers*Number of molecules5
Total formula weight242.5
All*Total formula weight140426.3
*Water molecules are not included.

247947

PDB entries from 2026-01-21

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