8XZV
Architecture of the spinach plastid-encoded RNA polymerase
Summary for 8XZV
| Entry DOI | 10.2210/pdb8xzv/pdb |
| EMDB information | 38799 |
| Descriptor | DNA-directed RNA polymerase subunit alpha, pTAC6, superoxide dismutase, ... (18 entities in total) |
| Functional Keywords | plastid-encoded rna polymerase, chloroplast, spinach, transcription |
| Biological source | Spinacia oleracea (spinach) More |
| Total number of polymer chains | 19 |
| Total formula weight | 1112543.21 |
| Authors | |
| Primary citation | Wang, T.,Wang, G.L.,Fang, Y.,Zhang, Y.,Peng, W.,Zhou, Y.,Zhang, A.,Yu, L.J.,Lu, C. Architecture of the spinach plastid-encoded RNA polymerase. Nat Commun, 15:9838-9838, 2024 Cited by PubMed Abstract: The plastid-encoded RNA polymerase serves as the principal transcription machinery within chloroplasts, transcribing over 80% of all primary plastid transcripts. This polymerase consists of a prokaryotic-like core enzyme known as the plastid-encoded RNA polymerase core, and is supplemented by newly evolved associated proteins known as PAPs. However, the architecture of the plastid-encoded RNA polymerase and the possible functions of PAPs remain unknown. Here, we present the cryo-electron microscopy structure of a 19-subunit plastid-encoded RNA polymerase complex derived from spinach (Spinacia oleracea). The structure shows that the plastid-encoded RNA polymerase core resembles bacterial RNA polymerase. Twelve PAPs and two additional proteins (FLN2 and pTAC18) bind at the periphery of the plastid-encoded RNA polymerase core, forming extensive interactions that may facilitate complex assembly and stability. PAPs may also protect the complex against oxidative damage and has potential functions in transcriptional regulation. This research offers a structural basis for future investigations into the functions and regulatory mechanisms governing the transcription of plastid genes. PubMed: 39537621DOI: 10.1038/s41467-024-54266-2 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.16 Å) |
Structure validation
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