8XKV

Cryo-EM structure of the Ycf2-FtsHi motor complex from Arabidopsis in Apo state

Summary for 8XKV

• Entry DOI: 10.2210/pdb8xkv/pdb
• EMDB information: 38425 38426 38427 38428
• Descriptor: Probable inactive ATP-dependent zinc metalloprotease FTSHI 4, chloroplastic, Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B, UNK, ... (18 entities in total)
• Functional Keywords: atp-motor, ycf2, ftshi, membrane protein
• Biological source: Arabidopsis thaliana (thale cress); More
• Total number of polymer chains: 17
• Total formula weight: 1067463.07

Authors

Liang, K.,Zhan, X.,Xu, Q.,Wu, J.,Yan, Z. (deposition date: 2023-12-25, release date: 2024-09-11, Last modification date: 2024-10-23)

Primary citation

Liang, K.,Jin, Z.,Zhan, X.,Li, Y.,Xu, Q.,Xie, Y.,Yang, Y.,Wang, S.,Wu, J.,Yan, Z.
Structural insights into the chloroplast protein import in land plants.
Cell, 187:5651-, 2024

PubMed Abstract: Chloroplast proteins are imported via the translocon at the outer chloroplast membrane (TOC)-translocon at the inner chloroplast membrane (TIC) supercomplex, driven by an ATPase motor. The Ycf2-FtsHi complex has been identified as the chloroplast import motor. However, its assembly and cooperation with the TIC complex during preprotein translocation remain unclear. Here, we present the structures of the Ycf2-FtsHi and TIC complexes from Arabidopsis and an ultracomplex formed between them from Pisum. The Ycf2-FtsHi structure reveals a heterohexameric AAA+ ATPase motor module with characteristic features. Four previously uncharacterized components of Ycf2-FtsHi were identified, which aid in complex assembly and anchoring of the motor module at a tilted angle relative to the membrane. When considering the structures of the TIC complex and the TIC-Ycf2-FtsHi ultracomplex together, it becomes evident that the tilted motor module of Ycf2-FtsHi enables its close contact with the TIC complex, thereby facilitating efficient preprotein translocation. Our study provides valuable structural insights into the chloroplast protein import process in land plants.

PubMed: 39197452
DOI: 10.1016/j.cell.2024.08.003

Experimental method

ELECTRON MICROSCOPY (3.3 Å)