8XDD

Cryo-EM structure of human urea transporter A2.

Summary for 8XDD

• Entry DOI: 10.2210/pdb8xdd/pdb
• EMDB information: 38274
• Descriptor: Urea transporter 2, 8-hydroxyquinoline-2-carboxylic acid, 1-(3-methoxyphenyl)methanamine (3 entities in total)
• Functional Keywords: urea transporter, membrane protein
• Biological source: Homo sapien
• Total number of polymer chains: 3
• Total formula weight: 131238.40

Authors

Huang, S.,Liu, L.,Sun, J. (deposition date: 2023-12-10, release date: 2024-12-04)

Primary citation

Huang, S.M.,Huang, Z.Z.,Liu, L.,Xiong, M.Y.,Zhang, C.,Cai, B.Y.,Wang, M.W.,Cai, K.,Jia, Y.L.,Wang, J.L.,Zhang, M.H.,Xie, Y.H.,Li, M.,Zhang, H.,Weng, C.H.,Wen, X.,Li, Z.,Sun, Y.,Yi, F.,Yang, Z.,Xiao, P.,Yang, F.,Yu, X.,Tie, L.,Yang, B.X.,Sun, J.P.
Structural insights into the mechanisms of urea permeation and distinct inhibition modes of urea transporters.
Nat Commun, 15:10226-10226, 2024

PubMed Abstract: Urea's transmembrane transport through urea transporters (UT) is a fundamental physiological behavior for life activities. Here, we present 11 cryo-EM structures of four UT members in resting states, urea transport states, or inactive states bound with synthetic competitive, uncompetitive or noncompetitive inhibitor. Our results indicate that the binding of urea via a conserved urea recognition motif (URM) and the urea transport via H-bond transfer along the Q-T-T-Q motif among different UT members. Moreover, distinct binding modes of the competitive inhibitors 25a and ATB3, the uncompetitive inhibitor CF11 and the noncompetitive inhibitor HQA2 provide different mechanisms for blocking urea transport and achieved selectivity through L-P pocket, UCBP region and SCG pocket, respectively. In summary, our study not only allows structural understanding of urea transport via UTs but also afforded a structural landscape of hUT-A2 inhibition by competitive, uncompetitive and noncompetitive inhibitors, which may facilitate developing selective human UT-A inhibitors as a new class of salt-sparing diuretics.

PubMed: 39587082
DOI: 10.1038/s41467-024-54305-y

Experimental method

ELECTRON MICROSCOPY (3 Å)