8X7N
Cell-cell adhesion Nanobody/Antigen Pair
Summary for 8X7N
| Entry DOI | 10.2210/pdb8x7n/pdb |
| Descriptor | Conjugative transfer: aggregate stability, Nb-TraN (2 entities in total) |
| Functional Keywords | bacterial cell adhesion, cell targeting, nanobodies, membrane protein, synthetic biology, antimicrobial protein |
| Biological source | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 More |
| Total number of polymer chains | 10 |
| Total formula weight | 139049.55 |
| Authors | Chen, P.-P.,Hsia, K.-C.,Ting, S.-Y.,Chen, Y.-C. (deposition date: 2023-11-24, release date: 2024-07-17, Last modification date: 2026-01-28) |
| Primary citation | Chen, P.Y.,Chen, Y.C.,Chen, P.P.,Lin, K.T.,Sargsyan, K.,Hsu, C.P.,Wang, W.L.,Hsia, K.C.,Ting, S.Y. A whole-cell platform for discovering synthetic cell adhesion molecules in bacteria. Nat Commun, 15:6568-6568, 2024 Cited by PubMed Abstract: Developing programmable bacterial cell-cell adhesion is of significant interest due to its versatile applications. Current methods that rely on presenting cell adhesion molecules (CAMs) on bacterial surfaces are limited by the lack of a generalizable strategy to identify such molecules targeting bacterial membrane proteins in their natural states. Here, we introduce a whole-cell screening platform designed to discover CAMs targeting bacterial membrane proteins within a synthetic bacteria-displayed nanobody library. Leveraging the potency of the bacterial type IV secretion system-a contact-dependent DNA delivery nanomachine-we have established a positive feedback mechanism to selectively enrich for bacteria displaying nanobodies that target antigen-expressing cells. Our platform successfully identified functional CAMs capable of recognizing three distinct outer membrane proteins (TraN, OmpA, OmpC), demonstrating its efficacy in CAM discovery. This approach holds promise for engineering bacterial cell-cell adhesion, such as directing the antibacterial activity of programmed inhibitor cells toward target bacteria in mixed populations. PubMed: 39095377DOI: 10.1038/s41467-024-51017-1 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.67 Å) |
Structure validation
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