8X7N
Cell-cell adhesion Nanobody/Antigen Pair
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 07A |
Synchrotron site | NSRRC |
Beamline | TPS 07A |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2023-11-02 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 96.864, 153.263, 163.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.850 - 3.670 |
R-factor | 0.2615 |
Rwork | 0.260 |
R-free | 0.28540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vnv |
RMSD bond length | 0.002 |
RMSD bond angle | 0.478 |
Data scaling software | HKL-2000 (v7.22) |
Phasing software | PHENIX (1.19.2_4158) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.850 | 3.830 |
High resolution limit [Å] | 3.670 | 3.670 |
Number of reflections | 26580 | 2604 |
<I/σ(I)> | 7.3 | |
Completeness [%] | 96.5 | |
Redundancy | 8.6 | |
CC(1/2) | 0.988 | 0.955 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Sodium acetate pH 4.6, 1.0 M Ammonium sulfate |