8X1I

ParM present of genome of Desufitobacterium hafniense - Dh-cParM1

Summary for 8X1I

• Entry DOI: 10.2210/pdb8x1i/pdb
• EMDB information: 37996
• Descriptor: ParM present of genome of Desufitobacterium hafniense - Dh-cParM1, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION (3 entities in total)
• Functional Keywords: parm, genome, segregation, plasmid, structural protein
• Biological source: Desulfitobacterium hafniense Y51
• Total number of polymer chains: 1
• Total formula weight: 41043.65

Authors

Ali, S.,Robinson, R.C.,Narita, A. (deposition date: 2023-11-07, release date: 2024-11-13, Last modification date: 2025-07-16)

Primary citation

Ali, S.,Koh, A.,Popp, D.,Tanaka, K.,Kitaoku, Y.,Miyazaki, N.,Iwasaki, K.,Mitsuoka, K.,Robinson, R.C.,Narita, A.
Bacterial genome-encoded ParMs.
J.Biol.Chem., 301:110351-110351, 2025

PubMed Abstract: ParMs generally exist on low-copy number plasmids where they contribute to plasmid segregation and stable inheritance. We carried out bioinformatics analysis, which indicated that ParM genes are not only confined to plasmids but are also occasionally found on genomes. Here we report the discovery and characterization of two chromosome-encoded ParMs (cParMs) from the genomes of Desulfitobacterium hafniense (Dh-cParM1) and Clostridium botulinum (Cb-cParM). Both cParMs form filaments, exhibit nucleotide hydrolysis, and possess characteristic ParM subunit structures. Dh-cParM1 forms single and tightly coupled double filaments and is highly conserved on the chromosomes of five of six Desulfitobacterium species. Interestingly, these bacteria have not been reported to harbor plasmids. Cb-cParM possesses unique properties. Its filaments were stable after nucleotide hydrolysis and Pi release, and its ParR (Cb-cParR) did not affect the initial phase of Cb-cParM polymerization but displayed properties of a depolymerization factor for mature filaments. These results indicate functional, polymerizing ParMs can be encoded on genomes, suggesting that ParM roles may extend to other functions beyond plasmid segregation.

PubMed: 40499760
DOI: 10.1016/j.jbc.2025.110351

Experimental method

ELECTRON MICROSCOPY (4 Å)