8WXE

Vgamma5Vdelta1 EH TCR-CD3 complex

Summary for 8WXE

• Entry DOI: 10.2210/pdb8wxe/pdb
• EMDB information: 37904
• Descriptor: T-cell surface glycoprotein CD3 zeta chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, ... (7 entities in total)
• Functional Keywords: immunity, receptor, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 8
• Total formula weight: 201820.13

Authors

Xin, W.,Huang, B.,Chi, X.,Xu, M.,Zhang, Y.,Li, X.,Su, Q.,Zhou, Q. (deposition date: 2023-10-28, release date: 2024-05-08, Last modification date: 2024-06-19)

Primary citation

Xin, W.,Huang, B.,Chi, X.,Liu, Y.,Xu, M.,Zhang, Y.,Li, X.,Su, Q.,Zhou, Q.
Structures of human gamma delta T cell receptor-CD3 complex.
Nature, 630:222-229, 2024

PubMed Abstract: Gamma delta (γδ) T cells, a unique T cell subgroup, are crucial in various immune responses and immunopathology. The γδ T cell receptor (TCR), which is generated by γδ T cells, recognizes a diverse range of antigens independently of the major histocompatibility complex. The γδ TCR associates with CD3 subunits, initiating T cell activation and holding great potential in immunotherapy. Here we report the structures of two prototypical human Vγ9Vδ2 and Vγ5Vδ1 TCR-CD3 complexes, revealing two distinct assembly mechanisms that depend on Vγ usage. The Vγ9Vδ2 TCR-CD3 complex is monomeric, with considerable conformational flexibility in the TCRγ-TCRδ extracellular domain and connecting peptides. The length of the connecting peptides regulates the ligand association and T cell activation. A cholesterol-like molecule wedges into the transmembrane region, exerting an inhibitory role in TCR signalling. The Vγ5Vδ1 TCR-CD3 complex displays a dimeric architecture, whereby two protomers nestle back to back through the Vγ5 domains of the TCR extracellular domains. Our biochemical and biophysical assays further corroborate the dimeric structure. Importantly, the dimeric form of the Vγ5Vδ1 TCR is essential for T cell activation. These findings reveal organizing principles of the γδ TCR-CD3 complex, providing insights into the unique properties of γδ TCR and facilitating immunotherapeutic interventions.

PubMed: 38657677
DOI: 10.1038/s41586-024-07439-4

Experimental method

ELECTRON MICROSCOPY (4 Å)