8WDE
CryoEM structure of the spike protein of human CoV 229E in complex with receptor hAPN (composite map)
Summary for 8WDE
| Entry DOI | 10.2210/pdb8wde/pdb |
| EMDB information | 37462 |
| Descriptor | Spike glycoprotein, Aminopeptidase N, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total) |
| Functional Keywords | human aminopeptidase n, viral protein |
| Biological source | Human coronavirus 229E More |
| Total number of polymer chains | 5 |
| Total formula weight | 607441.33 |
| Authors | Hsu, S.T.D.,Tsai, Y.X. (deposition date: 2023-09-15, release date: 2025-03-05, Last modification date: 2025-03-12) |
| Primary citation | Tsai, Y.X.,Chien, Y.C.,Hsu, M.F.,Khoo, K.H.,Hsu, S.D. Molecular basis of host recognition of human coronavirus 229E. Nat Commun, 16:2045-2045, 2025 Cited by PubMed Abstract: Human coronavirus 229E (HCoV-229E) is the earliest CoV found to infect humans. It binds to the human aminopeptidase N (hAPN) through the receptor binding domain (RBD) of its spike (S) protein to achieve host recognition. We present the cryo-electron microscopy structure of two HCoV-229E S protein in complex with a dimeric hAPN to provide structural insights on how the HCoV-229E S protein opens up its RBD to engage with its host receptor, information that is currently missing among alphacoronaviruses to which HCoV-229E belong. We quantitatively profile the glycosylation of HCoV-229E S protein and hAPN to deduce the glyco-shielding effects pertinent to antigenicity and host recognition. Finally, we present an atomic model of fully glycosylated HCoV-229E S in complex with hAPN anchored on their respective membrane bilayers to recapitulate the structural basis of the first step of host infection by HCoV-229E. PubMed: 40016196DOI: 10.1038/s41467-025-57359-8 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.6 Å) |
Structure validation
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