8W8E

human co-transcriptional RNA capping enzyme RNGTT

Summary for 8W8E

• Entry DOI: 10.2210/pdb8w8e/pdb
• EMDB information: 37352
• Descriptor: DNA-directed RNA polymerase subunit, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11-a, ... (25 entities in total)
• Functional Keywords: rna polymerase ii, capping, pausing, transcription
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 22
• Total formula weight: 988374.97

Authors

Li, Y.,Wang, Q.,Xu, Y.,Li, Z. (deposition date: 2023-09-02, release date: 2024-04-10, Last modification date: 2024-06-19)

Primary citation

Li, Y.,Wang, Q.,Xu, Y.,Li, Z.
Structures of co-transcriptional RNA capping enzymes on paused transcription complex.
Nat Commun, 15:4622-4622, 2024

PubMed Abstract: The 5'-end capping of nascent pre-mRNA represents the initial step in RNA processing, with evidence demonstrating that guanosine addition and 2'-O-ribose methylation occur in tandem with early steps of transcription by RNA polymerase II, especially at the pausing stage. Here, we determine the cryo-EM structures of the paused elongation complex in complex with RNGTT, as well as the paused elongation complex in complex with RNGTT and CMTR1. Our findings show the simultaneous presence of RNGTT and the NELF complex bound to RNA polymerase II. The NELF complex exhibits two conformations, one of which shows a notable rearrangement of NELF-A/D compared to that of the paused elongation complex. Moreover, CMTR1 aligns adjacent to RNGTT on the RNA polymerase II stalk. Our structures indicate that RNGTT and CMTR1 directly bind the paused elongation complex, illuminating the mechanism by which 5'-end capping of pre-mRNA during transcriptional pausing.

PubMed: 38816438
DOI: 10.1038/s41467-024-48963-1

Experimental method

ELECTRON MICROSCOPY (3.9 Å)