8W8E
human co-transcriptional RNA capping enzyme RNGTT
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | DNA-directed RNA polymerase subunit | polymer | 1970 | 217450.1 | 1 | UniProt (A0A8D1DPV6) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) In PDB | Sus scrofa (pig) | |
| 2 | B | DNA-directed RNA polymerase subunit beta | polymer | 1174 | 134041.4 | 1 | UniProt (A0A4X1TVZ5) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) In PDB | Sus scrofa (pig) | |
| 3 | C | DNA-directed RNA polymerase II subunit RPB3 | polymer | 271 | 30997.6 | 1 | UniProt (A0A481DF93) Pfam (PF01193) Pfam (PF01000) In PDB | Sus scrofa (pig) | DNA-directed RNA polymerase II subunit C |
| 4 | D | DNA-directed RNA polymerase II subunit RPB4 | polymer | 142 | 16331.3 | 1 | UniProt (A0A481BYI6) Pfam (PF03874) In PDB | Sus scrofa (pig) | DNA-directed RNA polymerase II subunit RPB4 isoform X1, DNA-directed RNA polymerase II subunit D |
| 5 | E | DNA-directed RNA polymerase II subunit E | polymer | 210 | 24644.3 | 1 | UniProt (A0A4X1VTX4) Pfam (PF03871) Pfam (PF01191) In PDB | Sus scrofa (pig) | RPB5 homolog |
| 6 | F | DNA-directed RNA polymerase II subunit F | polymer | 127 | 14477.0 | 1 | UniProt (A0A4X1VEK9) Pfam (PF01192) In PDB | Sus scrofa (pig) | DNA-directed RNA polymerases I,II,and III subunit RPABC2,RPB6 homolog |
| 7 | G | DNA-directed RNA polymerase II subunit RPB7 | polymer | 172 | 19314.3 | 1 | UniProt (A0A4X1VKG7) Pfam (PF03876) Pfam (PF00575) In PDB | Sus scrofa (pig) | DNA-directed RNA polymerase II subunit G |
| 8 | H | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 150 | 17162.3 | 1 | UniProt (I3LCB2) Pfam (PF03870) In PDB | Sus scrofa (pig) | |
| 9 | I | DNA-directed RNA polymerase II subunit RPB9 | polymer | 125 | 14541.2 | 1 | UniProt (P60899) Pfam (PF02150) Pfam (PF01096) In PDB | Sus scrofa (pig) | RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5 |
| 10 | J | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 67 | 7655.1 | 1 | UniProt (A0A493TD97) Pfam (PF01194) In PDB | Sus scrofa (pig) | |
| 11 | K | DNA-directed RNA polymerase II subunit RPB11-a | polymer | 117 | 13310.3 | 1 | UniProt (F1RKE4) Pfam (PF13656) Pfam (PF01193) In PDB | Sus scrofa (pig) | |
| 12 | L | RPB12 | polymer | 58 | 7018.2 | 1 | UniProt (A0A4X1TRS6) Pfam (PF03604) In PDB | Sus scrofa (pig) | |
| 13 | N | DNA (36-MER) | polymer | 48 | 14932.5 | 1 | Homo sapiens | ||
| 14 | P | RNA (5'-D(*(GTP))-R(P*AP*GP*AP*GP*AP*GP*GP*GP*AP*AP*CP*CP*CP*AP*CP*U)-3') | polymer | 16 | 5183.2 | 1 | Homo sapiens | ||
| 15 | T | DNA (45-MER) | polymer | 48 | 14672.3 | 1 | Homo sapiens | ||
| 16 | U | Negative elongation factor A | polymer | 528 | 57343.6 | 1 | UniProt (Q9H3P2) In PDB | Homo sapiens (human) | NELF-A,Wolf-Hirschhorn syndrome candidate 2 protein |
| 17 | V | Negative elongation factor B | polymer | 580 | 65779.2 | 1 | UniProt (Q8WX92) Pfam (PF06209) In PDB | Homo sapiens (human) | NELF-B,Cofactor of BRCA1 |
| 18 | W | Negative elongation factor C/D | polymer | 584 | 65819.7 | 1 | UniProt (Q8IXH7) Pfam (PF04858) In PDB | Homo sapiens (human) | |
| 19 | X | Negative elongation factor E | polymer | 380 | 43320.9 | 1 | UniProt (P18615) Pfam (PF00076) In PDB | Homo sapiens (human) | NELF-E,RNA-binding protein RD |
| 20 | Y | Transcription elongation factor SPT4 | polymer | 121 | 13508.5 | 1 | UniProt (P63272) Pfam (PF06093) In PDB | Homo sapiens (human) | hSPT4,DRB sensitivity-inducing factor 14 kDa subunit,DSIF p14,DRB sensitivity-inducing factor small subunit,DSIF small subunit |
| 21 | Z | Transcription elongation factor SPT5 | polymer | 1087 | 121145.5 | 1 | UniProt (O00267) Pfam (PF11942) Pfam (PF03439) Pfam (PF00467) In PDB | Homo sapiens (human) | hSPT5,DRB sensitivity-inducing factor 160 kDa subunit,DSIF p160,DRB sensitivity-inducing factor large subunit,DSIF large subunit,Tat-cotransactivator 1 protein,Tat-CT1 protein |
| 22 | a | mRNA-capping enzyme | polymer | 597 | 68655.7 | 1 | UniProt (O60942) Pfam (PF00782) Pfam (PF01331) Pfam (PF03919) In PDB | Homo sapiens (human) | |
| 23 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 24 | C, I, L, Y, A... | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 25 | P | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) |
Sequence modifications
W: 10 - 590 (UniProt: Q8IXH7)
Y: 1 - 117 (UniProt: P63272)
| PDB | External Database | Details |
|---|---|---|
| Ser 7 | - | expression tag |
| Asn 8 | - | expression tag |
| Ala 9 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 22 |
| Total formula weight | 987304.2 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1070.8 | |
| All* | Total formula weight | 988375.0 |
