8VKH

Structure of gelsolin domains G1G3 bound to the barbed end of F-actin

8VKH の概要

• エントリーDOI: 10.2210/pdb8vkh/pdb
• 関連するPDBエントリー: 8VIZ
• EMDBエントリー: 43274 43316
• 分子名称: Actin, alpha skeletal muscle, Gelsolin, ADENOSINE-5'-DIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: cytoskeleton, actin, cell motility, protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 419751.02

構造登録者

Barrie, K.R.,Rebowski, G.,Dominguez, R. (登録日: 2024-01-09, 公開日: 2024-10-16, 最終更新日: 2025-05-21)

主引用文献

Barrie, K.R.,Rebowski, G.,Dominguez, R.
Mechanism of actin filament severing and capping by gelsolin.
Nat.Struct.Mol.Biol., 32:237-242, 2025

PubMed Abstract: Gelsolin is the prototypical member of a family of Ca-activated F-actin severing and capping proteins. Here we report structures of Ca-bound human gelsolin at the barbed end of F-actin. One structure reveals gelsolin's six domains (G1G6) and interdomain linkers wrapping around F-actin, while another shows domains G1G3-a fragment observed during apoptosis-binding on both sides of F-actin. Conformational changes that trigger severing occur on one side of F-actin with G1G6 and on both sides with G1G3. Gelsolin remains bound after severing, blocking subunit exchange.

PubMed: 39448849
DOI: 10.1038/s41594-024-01412-5

実験手法

ELECTRON MICROSCOPY (3.63 Å)