8VJ2
Crystal Structure of Macrophage migration inhibitory factor-1 (MIF1) from Onchocerca volvulus
Summary for 8VJ2
| Entry DOI | 10.2210/pdb8vj2/pdb |
| Descriptor | Macrophage migration inhibitory factor-1, SODIUM ION (3 entities in total) |
| Functional Keywords | ssgcid, structural genomics, seattle structural genomics center for infectious disease, macrophage migration inhibitory factor-1, cytokine |
| Biological source | Onchocerca volvulus |
| Total number of polymer chains | 3 |
| Total formula weight | 43398.36 |
| Authors | Seattle Structural Genomics Center for Infectious Disease,Seattle Structural Genomics Center for Infectious Disease (SSGCID) (deposition date: 2024-01-05, release date: 2024-01-17, Last modification date: 2024-12-18) |
| Primary citation | Kimble, A.D.,Dawson, O.C.O.,Liu, L.,Subramanian, S.,Cooper, A.,Battaile, K.,Craig, J.,Harmon, E.,Myler, P.,Lovell, S.,Asojo, O.A. Crystal structure of N-terminally hexahistidine-tagged Onchocerca volvulus macrophage migration inhibitory factor-1. Acta Crystallogr.,Sect.F, 80:328-334, 2024 Cited by PubMed Abstract: Onchocerca volvulus causes blindness, onchocerciasis, skin infections and devastating neurological diseases such as nodding syndrome. New treatments are needed because the currently used drug, ivermectin, is contraindicated in pregnant women and those co-infected with Loa loa. The Seattle Structural Genomics Center for Infectious Disease (SSGCID) produced, crystallized and determined the apo structure of N-terminally hexahistidine-tagged O. volvulus macrophage migration inhibitory factor-1 (His-OvMIF-1). OvMIF-1 is a possible drug target. His-OvMIF-1 has a unique jellyfish-like structure with a prototypical macrophage migration inhibitory factor (MIF) trimer as the `head' and a unique C-terminal `tail'. Deleting the N-terminal tag reveals an OvMIF-1 structure with a larger cavity than that observed in human MIF that can be targeted for drug repurposing and discovery. Removal of the tag will be necessary to determine the actual biological oligomer of OvMIF-1 because size-exclusion chomatographic analysis of His-OvMIF-1 suggests a monomer, while PISA analysis suggests a hexamer stabilized by the unique C-terminal tails. PubMed: 39503735DOI: 10.1107/S2053230X24010550 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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