8VJ2
Crystal Structure of Macrophage migration inhibitory factor-1 (MIF1) from Onchocerca volvulus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 19-ID |
Synchrotron site | NSLS-II |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-12-09 |
Detector | DECTRIS EIGER2 XE 9M |
Wavelength(s) | 0.9785 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 52.441, 91.127, 132.487 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.450 - 1.900 |
R-factor | 0.2176 |
Rwork | 0.217 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.700 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.21rc1_5177: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.450 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.087 | 1.095 |
Rmeas | 0.091 | 1.136 |
Rpim | 0.025 | 0.299 |
Total number of observations | 340455 | 25834 |
Number of reflections | 25433 | 1843 |
<I/σ(I)> | 15 | 2.1 |
Completeness [%] | 99.9 | |
Redundancy | 13.4 | 14 |
CC(1/2) | 0.999 | 0.956 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | Berkeley B3: 100 mM Bis-Tris / Hydrochloric acid pH 6.5 400 mM Sodium chloride 30% (w/v) PEG 3350, OnvoA.00834.a.UX1.PW39231 at 20.1 mg/mL. plate 13715 well B3 drop 2. Puck: PSL-1003, Cryo: crystallization solution |