8V6N

Open-state cryo-EM structure of human TRPV3 in presence of 2-APB in cNW30 nanodiscs

Summary for 8V6N

• Entry DOI: 10.2210/pdb8v6n/pdb
• Related: 8V6K 8V6L 8V6M
• EMDB information: 42994 42995 42996 42997
• Descriptor: Transient receptor potential cation channel subfamily V member 3, (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate, ARACHIDONIC ACID, ... (6 entities in total)
• Functional Keywords: transient receptor potential v family member 3, trp, channel, trpv3, trp channels, membrane protein, 2-aminoethoxydiphenylborane, 2-apb
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 4
• Total formula weight: 388963.19

Authors

Nadezhdin, K.D.,Neuberger, A.,Sobolevsky, A.I. (deposition date: 2023-12-01, release date: 2024-04-17, Last modification date: 2024-10-23)

Primary citation

Nadezhdin, K.D.,Neuberger, A.,Khosrof, L.S.,Talyzina, I.A.,Khau, J.,Yelshanskaya, M.V.,Sobolevsky, A.I.
TRPV3 activation by different agonists accompanied by lipid dissociation from the vanilloid site.
Sci Adv, 10:eadn2453-eadn2453, 2024

PubMed Abstract: TRPV3 represents both temperature- and ligand-activated transient receptor potential (TRP) channel. Physiologically relevant opening of TRPV3 channels by heat has been captured structurally, while opening by agonists has only been observed in structures of mutant channels. Here, we present cryo-EM structures that illuminate opening and inactivation of wild-type human TRPV3 in response to binding of two types of agonists: either the natural cannabinoid tetrahydrocannabivarin (THCV) or synthetic agonist 2-aminoethoxydiphenylborane (2-APB). We found that THCV binds to the vanilloid site, while 2-APB binds to the S1-S4 base and ARD-TMD linker sites. Despite binding to distally located sites, both agonists induce similar pore opening and cause dissociation of a lipid that occupies the vanilloid site in their absence. Our results uncover different but converging allosteric pathways through which small-molecule agonists activate TRPV3 and provide a framework for drug design and understanding the role of lipids in ion channel function.

PubMed: 38691614
DOI: 10.1126/sciadv.adn2453

Experimental method

ELECTRON MICROSCOPY (2.59 Å)