8UZL
Designed Transmembrane beta-barrel- TMB10_163
Summary for 8UZL
| Entry DOI | 10.2210/pdb8uzl/pdb |
| Descriptor | Designed Transmembrane beta-barrel TMB10_163, HEXANE-1,6-DIOL (3 entities in total) |
| Functional Keywords | computational design, de novo protein, nanopore, tmb |
| Biological source | synthetic construct |
| Total number of polymer chains | 4 |
| Total formula weight | 65989.78 |
| Authors | Bera, A.K.,Lemma, S.B.,Kang, A.,Baker, D. (deposition date: 2023-11-15, release date: 2024-07-17, Last modification date: 2024-07-31) |
| Primary citation | Berhanu, S.,Majumder, S.,Muntener, T.,Whitehouse, J.,Berner, C.,Bera, A.K.,Kang, A.,Liang, B.,Khan, N.,Sankaran, B.,Tamm, L.K.,Brockwell, D.J.,Hiller, S.,Radford, S.E.,Baker, D.,Vorobieva, A.A. Sculpting conducting nanopore size and shape through de novo protein design. Science, 385:282-288, 2024 Cited by PubMed Abstract: Transmembrane β-barrels have considerable potential for a broad range of sensing applications. Current engineering approaches for nanopore sensors are limited to naturally occurring channels, which provide suboptimal starting points. By contrast, de novo protein design can in principle create an unlimited number of new nanopores with any desired properties. Here we describe a general approach to designing transmembrane β-barrel pores with different diameters and pore geometries. Nuclear magnetic resonance and crystallographic characterization show that the designs are stably folded with structures resembling those of the design models. The designs have distinct conductances that correlate with their pore diameter, ranging from 110 picosiemens (~0.5 nanometer pore diameter) to 430 picosiemens (~1.1 nanometer pore diameter). Our approach opens the door to the custom design of transmembrane nanopores for sensing and sequencing applications. PubMed: 39024453DOI: 10.1126/science.adn3796 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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