8UZL
Designed Transmembrane beta-barrel- TMB10_163
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-05-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000040 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.628, 52.552, 100.050 |
| Unit cell angles | 90.00, 93.67, 90.00 |
Refinement procedure
| Resolution | 55.510 - 2.500 |
| R-factor | 0.2288 |
| Rwork | 0.227 |
| R-free | 0.26210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.440 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.510 | 2.580 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.126 | 0.923 |
| Rpim | 0.069 | 0.493 |
| Number of reflections | 38215 | 1961 |
| <I/σ(I)> | 4.26 | 0.36 |
| Completeness [%] | 98.9 | 98.54 |
| Redundancy | 4.3 | 4.5 |
| CC(1/2) | 0.990 | 0.832 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 3.25M 1,6 Hexanediol and 0.01 M HEPES pH 7.5 |
