8UZB

Cryo-EM structure of iGeoCas9 in complex with sgRNA and target DNA

Summary for 8UZB

• Entry DOI: 10.2210/pdb8uzb/pdb
• Related: 8UZA
• EMDB information: 42837 42838
• Descriptor: CRISPR-associated endonuclease Cas9, RNA (107-MER), Target strand DNA (39-MER), ... (4 entities in total)
• Functional Keywords: rna-guided dna endonuclease, ternary complex, crispr, hydrolase, hydrolase-rna-dna complex, hydrolase/rna/dna
• Biological source: Geobacillus stearothermophilus; More
• Total number of polymer chains: 4
• Total formula weight: 202904.34

Authors

Eggers, A.R.,Soczek, K.M.,Tuck, O.T.,Doudna, J.A. (deposition date: 2023-11-14, release date: 2024-05-29, Last modification date: 2024-07-03)

• Primary citation: Eggers, A.R.,Chen, K.,Soczek, K.M.,Tuck, O.T.,Doherty, E.E.,Xu, B.,Trinidad, M.I.,Thornton, B.W.,Yoon, P.H.,Doudna, J.A.; Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9.; Cell, 187:3249-, 2024; PubMed: 38781968; DOI: 10.1016/j.cell.2024.04.031

Experimental method

ELECTRON MICROSCOPY (2.63 Å)