8UZB
Cryo-EM structure of iGeoCas9 in complex with sgRNA and target DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated endonuclease Cas9 | polymer | 1087 | 127001.0 | 1 | UniProt (A0A150MP45) | Geobacillus stearothermophilus | |
| 2 | B (B) | RNA (107-MER) | polymer | 139 | 44488.1 | 1 | Geobacillus stearothermophilus | ||
| 3 | C (C) | Target strand DNA (39-MER) | polymer | 51 | 15797.2 | 1 | Mus musculus | ||
| 4 | D (D) | Non-target strand DNA | polymer | 51 | 15618.0 | 1 | Mus musculus |
Sequence modifications
A: 1 - 1087 (UniProt: A0A150MP45)
| PDB | External Database | Details |
|---|---|---|
| Ala 8 | Asp 8 | engineered mutation |
| Gly 149 | Glu 149 | engineered mutation |
| Ile 182 | Thr 182 | engineered mutation |
| Asp 206 | Asn 206 | engineered mutation |
| Gln 466 | Pro 466 | engineered mutation |
| Ala 582 | His 582 | engineered mutation |
| Arg 817 | Gln 817 | engineered mutation |
| Lys 843 | Glu 843 | engineered mutation |
| Gly 884 | Glu 884 | engineered mutation |
| Arg 908 | Lys 908 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 202904.3 | |
| All* | Total formula weight | 202904.3 |
