8UO7

Bovine trypsin in complex with deacetylated wild type microviridin J

Summary for 8UO7

Entry DOI	10.2210/pdb8uo7/pdb
Related PRD ID	PRD_002533
Descriptor	Cationic trypsin, Deacetylated wildtype microviridin J, SULFATE ION, ... (6 entities in total)
Functional Keywords	hydrolase inhibitor, hydrolase
Biological source	Bos taurus (cattle) More
Total number of polymer chains	2
Total formula weight	25251.35
Authors	Chen, W.,Bruner, S.D. (deposition date: 2023-10-19, release date: 2024-02-14, Last modification date: 2024-02-28)
Primary citation	Patel, K.P.,Chen, W.T.,Delbecq, L.,Bruner, S.D. Alternative Linkage Chemistries in the Chemoenzymatic Synthesis of Microviridin-Based Cyclic Peptides. Org.Lett., 26:1138-1142, 2024 Cited by PubMed Abstract: Engineering biosynthetic pathways to ribosomally synthesized and post-translationally modified peptides (RiPPs) offers several advantages for both and applications. Here we probe the ability of peptide cyclases to generate trimacrocycle microviridin analogs with non-native cross-links. The results demonstrate that diverse chemistries are tolerated by macrocyclases in the ATP-grasp family and allow for the construction of unique cyclic peptide architectures that retain protease inhibition activity. In addition, cocomplex structures of analogs bound to a model protease were determined, illustrating how changes in functional groups maintain peptide conformation and target binding. PubMed: 38306609 DOI: 10.1021/acs.orglett.3c04045 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.2 Å)

Structure validation