8UO7
Bovine trypsin in complex with deacetylated wild type microviridin J
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-07-06 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9201 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 71.650, 71.650, 72.976 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.550 - 2.200 |
R-factor | 0.19944 |
Rwork | 0.197 |
R-free | 0.25818 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | REFMAC (8.0.013) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.550 | 2.279 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.190 | 0.874 |
Rmeas | 0.201 | 0.923 |
Number of reflections | 11366 | 1120 |
<I/σ(I)> | 8.34 | |
Completeness [%] | 99.9 | 99.91 |
Redundancy | 9.8 | |
CC(1/2) | 0.991 | 0.883 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295.37 | 0.1 M sodium cacodylate pH 7.0, 0.2 M ammonium sulfate, 30%(w/v) PEG 8000 |