8UJA

T33-fn10 - Designed Tetrahedral Protein Cage Using Fragment-based Hydrogen Bond Networks

Summary for 8UJA

• Entry DOI: 10.2210/pdb8uja/pdb
• Descriptor: T33-fn10: engineered DrsE like sulfur reductase, T33-fn10: engineered enoyl-CoA hydratase/isomerase (2 entities in total)
• Functional Keywords: tetrahedral nanoparticle, designed protein, de novo interface, two-component complex, rosetta, de novo protein
• Biological source: Sulfurisphaera tokodaii str. 7; More
• Total number of polymer chains: 16
• Total formula weight: 318351.70

Authors

Meador, K.,Sawaya, M.R.,Yeates, T.O. (deposition date: 2023-10-11, release date: 2024-03-06, Last modification date: 2024-06-19)

Primary citation

Meador, K.,Castells-Graells, R.,Aguirre, R.,Sawaya, M.R.,Arbing, M.A.,Sherman, T.,Senarathne, C.,Yeates, T.O.
A suite of designed protein cages using machine learning and protein fragment-based protocols.
Structure, 32:751-765.e11, 2024

PubMed Abstract: Designed protein cages and related materials provide unique opportunities for applications in biotechnology and medicine, but their creation remains challenging. Here, we apply computational approaches to design a suite of tetrahedrally symmetric, self-assembling protein cages. For the generation of docked conformations, we emphasize a protein fragment-based approach, while for sequence design of the de novo interface, a comparison of knowledge-based and machine learning protocols highlights the power and increased experimental success achieved using ProteinMPNN. An analysis of design outcomes provides insights for improving interface design protocols, including prioritizing fragment-based motifs, balancing interface hydrophobicity and polarity, and identifying preferred polar contact patterns. In all, we report five structures for seven protein cages, along with two structures of intermediate assemblies, with the highest resolution reaching 2.0 Å using cryo-EM. This set of designed cages adds substantially to the body of available protein nanoparticles, and to methodologies for their creation.

PubMed: 38513658
DOI: 10.1016/j.str.2024.02.017

Experimental method

X-RAY DIFFRACTION (6 Å)