8UJA

T33-fn10 - Designed Tetrahedral Protein Cage Using Fragment-based Hydrogen Bond Networks

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I... (A, C, E, G, I...)	T33-fn10: engineered DrsE like sulfur reductase	polymer	108	12122.2	8		Sulfurisphaera tokodaii str. 7
2	B, D, F, H, J... (B, D, F, H, J...)	T33-fn10: engineered enoyl-CoA hydratase/isomerase	polymer	258	27671.7	8	UniProt (A4XEF6) Pfam (PF00378)	Novosphingobium aromaticivorans DSM 12444

Sequence modifications

B, D, F, H, J, L, N, P: 9 - 257 (UniProt: A4XEF6)

PDB	External Database	Details
Met 0	-	initiating methionine
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
Ser 7	-	expression tag
Gly 8	-	expression tag
Leu 87	Ala 79	engineered mutation
Thr 227	Ala 219	engineered mutation
Leu 228	Asp 220	engineered mutation
Cys 231	Arg 223	engineered mutation
Thr 237	Phe 229	engineered mutation
Leu 238	Glu 230	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	318351.7
All*	Total formula weight	318351.7

*Water molecules are not included.