8U48

Crystal structure of Bacteroides thetaiotamicron BT1285 D161A-E163A inactive Endoglycosidase in complex with high-mannose N-glycan (Man9GlcNAc2) substrate

Summary for 8U48

• Entry DOI: 10.2210/pdb8u48/pdb
• Descriptor: Endo-beta-N-acetylglucosaminidase, alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, PHOSPHATE ION, ... (4 entities in total)
• Functional Keywords: inactive endoglycosidase, artificial lectin, high-mannose specific, bacteroides, gh18 family, carbohydrate
• Biological source: Bacteroides thetaiotaomicron VPI-5482
• Total number of polymer chains: 2
• Total formula weight: 64854.31

Authors

Sastre, D.E.,Sultana, N.,Navarro, M.V.A.S.,Sundberg, E.J. (deposition date: 2023-09-09, release date: 2024-05-29, Last modification date: 2024-06-26)

• Primary citation: Sastre, D.E.,Sultana, N.,V A S Navarro, M.,Huliciak, M.,Du, J.,Cifuente, J.O.,Flowers, M.,Liu, X.,Lollar, P.,Trastoy, B.,Guerin, M.E.,Sundberg, E.J.; Human gut microbes express functionally distinct endoglycosidases to metabolize the same N-glycan substrate.; Nat Commun, 15:5123-5123, 2024; PubMed: 38879612; DOI: 10.1038/s41467-024-48802-3

Experimental method

X-RAY DIFFRACTION (1.9 Å)