8U48
Crystal structure of Bacteroides thetaiotamicron BT1285 D161A-E163A inactive Endoglycosidase in complex with high-mannose N-glycan (Man9GlcNAc2) substrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Endo-beta-N-acetylglucosaminidase | polymer | 282 | 30353.5 | 2 | UniProt (Q8A889) Pfam (PF00704) In PDB | Bacteroides thetaiotaomicron VPI-5482 | |
| 2 | C, D | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1883.7 | 2 | In PDB GlyTouCan (G60230HH) | |||
| 3 | A, B | PHOSPHATE ION | non-polymer | 95.0 | 4 | Chemie (PO4) | |||
| 4 | water | water | 18.0 | 645 | Chemie (HOH) |
Sequence modifications
A, B: 25 - 306 (UniProt: Q8A889)
| PDB | External Database | Details |
|---|---|---|
| Ala 161 | Asp 161 | engineered mutation |
| Ala 163 | Glu 163 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 60707.1 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 3767.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 379.9 | |
| All* | Total formula weight | 64854.3 |
