8U2C

Gaussian mixture model based single particle refinement - ABC transporter (inhibitor-bound ABCG2 from EMPIAR-10374)

Summary for 8U2C

Entry DOI	10.2210/pdb8u2c/pdb
Related	6ETI
EMDB information	3953 41845
Descriptor	Broad substrate specificity ATP-binding cassette transporter ABCG2, 5D3 Fab light chain variable domain, 5D3 Fab heavy chain variable domain, ... (5 entities in total)
Functional Keywords	abc transporter, membrane protein
Biological source	Homo sapiens (human) More
Total number of polymer chains	10
Total formula weight	331353.39
Authors	Chen, M.,Pintilie, G. (deposition date: 2023-09-05, release date: 2023-11-15, Last modification date: 2024-10-09)
Primary citation	Chen, M.,Schmid, M.F.,Chiu, W. Improving resolution and resolvability of single-particle cryoEM structures using Gaussian mixture models. Nat.Methods, 21:37-40, 2024 Cited by PubMed Abstract: Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here we developed a refinement protocol based on Gaussian mixture models that integrates particle orientation and conformation estimation and improves the alignment for flexible domains of protein structures. We demonstrated this protocol on multiple datasets, resulting in improved resolution and resolvability, locally and globally, by visual and quantitative measures. PubMed: 37973972 DOI: 10.1038/s41592-023-02082-9 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (2.5 Å)

Structure validation