8TS4

Crystal structure of T. Brucei hypoxanthine guanine phosphoribosyltransferase in complex with [2S,4S]-4-Guanin-9-yl-2-(2-phosphonoethoxymethyl)-1-N-(3-phosphonopropionyl)pyrrolidine

8TS4 の概要

• エントリーDOI: 10.2210/pdb8ts4/pdb
• 分子名称: Hypoxanthine-guanine phosphoribosyltransferase, MAGNESIUM ION, (3-{(2S,4S)-4-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)-2-[(2-phosphonoethoxy)methyl]pyrrolidin-1-yl}-3-oxopropyl)phosphonic acid, ... (4 entities in total)
• 機能のキーワード: inhibitor, complex, purine base, product analog, transferase
• 由来する生物種: Trypanosoma brucei
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47823.06

構造登録者

Guddat, L.W.,Guddat, L.W. (登録日: 2023-08-10, 公開日: 2024-05-08, 最終更新日: 2024-05-22)

主引用文献

Keough, D.T.,Petrova, M.,King, G.,Kratochvil, M.,Pohl, R.,Dolezelova, E.,Zikova, A.,Guddat, L.W.,Rejman, D.
Development of Prolinol Containing Inhibitors of Hypoxanthine-Guanine-Xanthine Phosphoribosyltransferase: Rational Structure-Based Drug Design.
J.Med.Chem., 67:7158-7175, 2024

PubMed Abstract: Inhibition of hypoxanthine-guanine-xanthine phosphoribosyltransferase activity decreases the pool of 6-oxo and 6-amino purine nucleoside monophosphates required for DNA and RNA synthesis, resulting in a reduction in cell growth. Therefore, inhibitors of this enzyme have potential to control infections, caused by and , , , and . Five compounds synthesized here that contain a purine base covalently linked by a prolinol group to one or two phosphonate groups have values ranging from 3 nM to >10 μM, depending on the structure of the inhibitor and the biological origin of the enzyme. X-ray crystal structures show that, on binding, these prolinol-containing inhibitors stimulated the movement of active site loops in the enzyme. Against in cell culture, a prodrug exhibited an EC of 10 μM. Thus, these compounds are excellent candidates for further development as drug leads against infectious diseases as well as being potential anticancer agents.

PubMed: 38651522
DOI: 10.1021/acs.jmedchem.4c00021

実験手法

X-RAY DIFFRACTION (2.2 Å)