8TPU
Subtomogram averaged consensus structure of the malarial 80S ribosome in Plasmodium falciparum-infected human erythrocytes
This is a non-PDB format compatible entry.
Summary for 8TPU
| Entry DOI | 10.2210/pdb8tpu/pdb |
| EMDB information | 41485 |
| Descriptor | 40S ribosomal protein S24, 40S ribosomal protein SA, 40S ribosomal protein S3, ... (79 entities in total) |
| Functional Keywords | ribosome, malaria, cryoet, in situ, plasmodium falciparum, translation |
| Biological source | Plasmodium falciparum 3D7 More |
| Total number of polymer chains | 79 |
| Total formula weight | 3419265.09 |
| Authors | |
| Primary citation | Anton, L.,Cheng, W.,Haile, M.T.,Dziekan, J.M.,Cobb, D.W.,Zhu, X.,Han, L.,Li, E.,Nair, A.,Lee, C.L.,Wang, H.,Ke, H.,Zhang, G.,Doud, E.H.,Cowman, A.F.,Ho, C.M. Integrated structural biology of the native malarial translation machinery and its inhibition by an antimalarial drug. Nat.Struct.Mol.Biol., 32:2158-2164, 2025 Cited by PubMed Abstract: Our understanding of cellular events is hampered by the gap between the resolution at which we can observe events inside cells and our ability to replicate physiological conditions in test tubes. Here, we show in Plasmodium falciparum, a non-model organism of high medical importance, that this gap can be bridged by using an integrated structural biology approach to visualize events inside the cell at molecular resolution. We determined eight high-resolution structures of the native malarial ribosome in actively translating states inside P. falciparum-infected human erythrocytes using in situ cryo-electron tomography. Following perturbation with a Plasmodium-specific translation inhibitor, we then observed a decrease in elongation factor-bound ribosomal states and an apparent upregulation of ribosome biogenesis in inhibitor-treated parasites. Our work elucidates new molecular details of the malarial translation elongation cycle and demonstrates direct multiscale visualization of drug-induced phenotypic changes in the structure and localization of individual molecules within the native cellular context. PubMed: 40825879DOI: 10.1038/s41594-025-01632-3 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (4.1 Å) |
Structure validation
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